5hmdc (Jena Bioscience)
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Structured Review
Jena Bioscience
5hmdc
5hmdc, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5-hydroxymethyl-dctp/5-Hydroxymethyl-dCTP/pmc12409352-115-10-13
Average 94 stars, based on 13 article reviews
5hmdc, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5-hydroxymethyl-dctp/5-Hydroxymethyl-dCTP/pmc12409352-115-10-13
Average 94 stars, based on 13 article reviews
5hmdc - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Modified DNA substrate selectivity by GmrSD-family Type IV restriction enzyme BrxU"
Article Title: Modified DNA substrate selectivity by GmrSD-family Type IV restriction enzyme BrxU
Journal: Philosophical Transactions of the Royal Society B: Biological Sciences
doi: 10.1098/rstb.2024.0072
Figure Legend Snippet: BrxU demonstrates preference between differing cytosine modifications. (A) Titrations of His 6 -BrxU were incubated with 100 ng of pUC19 incorporated with dC, 5hmdC, 5mdC or glc-5mdC for 10 min. The reactions were performed in triplicate, and representative gels are shown. The presence of ATP in the reaction is denoted with a + or − sign, and ATPγS is denoted by γS. (B) Timecourse of His 6 -BrxU (300 nM) mixed with 100 ng of pUC19 incorporated with dC, 5hmC, 5mC or glc-5hmC and incubated at 37 °C for from 1 to 30 min. The reactions were performed in triplicate, and representative gels are shown. The presence of ATP in the reaction is denoted with a + or − sign, and ATPγS is denoted by γS. (C) The average percentage of DNA remaining in His 6 -BrxU titration reactions with ATP compared with reactions without ATP, calculated from triplicate gels. Error bars represent standard deviation. (D) The average percentage DNA remaining in His 6 -BrxU timecourse reactions with ATP compared with reactions without ATP, calculated from triplicate gels. Error bars represent standard deviation.
Techniques Used: Incubation, Titration, Standard Deviation, Polymerase Chain Reaction
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